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   Athena Diagnostics   
      
   Notch3 (CADASIL) DNA Sequencing Test   
      
      
   Notch3 (CADASIL) DNA Sequencing Test   
   Order Test   
   Test Code   
   421   
   Test Details Technical Information CPT Coding Specimen Requirements Shipping   
   Considerations   
   Test Details   
   Test code:	421   
   Type of disorder:	Cerebrovascular Disease   
   Typical presentation:	Hereditary cause of stroke, dementia, migraine with   
   aura, mood disorders   
   Indications for testing:	Clinical features consistent with CADASIL   
   Disease(s) tested for:	CADASIL (Cerebral Autosomal Dominant Arteriopathy with   
   Subcortical Infarcts and Leukoencephalopathy)   
   Informed Consent Required:	Yes   
   Technical Information   
   Utility:	Detects sequence variants in the Notch3 gene   
   Methodology:	Next Generation Sequencing   
   Reference Value:	No sequence variants detected   
   CPT Coding   
   The CPT codes provided are based on AMA guidelines and are for informational   
   purposes only. CPT coding is the sole responsibility of the billing party.   
   Please direct any questions regarding coding to the payer being billed.   
      
   CPT:	81406(1)   
   Specimen Requirements   
   Please label each specimen tube with two forms of patient identification.   
   These forms of identification must also appear on the requisition form.   
      
   Specimen Type:	Whole blood   
   Specimen Stability:	Room temperature: 10 days, Refrigerated: 10 days, Frozen:   
   Unacceptable   
   Specimen Stability Notes:	DNA yields and quality are better in fresh   
   specimens. However, when necessary, most genetic tests are successful from DNA   
   purified from whole blood that is several days old.   
   Adult Preferred Volume:	8 mL   
   Adult Minimum Volume:	6 mL   
   Pediatric Preferred Volume:	2 mL   
   Pediatric Minimum Volume:	1 mL   
   Collection Tube:	Lavender top (EDTA)   
   Collection Notes:	Higher blood volumes ensure adequate DNA quantity, which   
   varies with WBC, specimen condition, and need for confirmatory testing.   
   Patients, 0-3 years have higher WBC, yielding more DNA per mL of blood   
   Shipping Considerations   
   Storage Conditions:	Refrigerate   
   Shipping Conditions:	Ship room temperature, avoid freezing.   
   Test Turnaround:	28-42 days   
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   and offers innovative tests for Alzheimer's disease, muscular dystrophy and   
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   Athena Diagnostics, Inc.   
   200 Forest Street, 2nd Floor   
   Marlborough, MA 01752   
      
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