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   Message 54,177 of 55,615   
   Ashu Seq to Poutnik   
   Re: Borate buffered Saline pH 8.4 (150 m   
   26 Sep 17 03:03:47   
   
   From: ashuseq2015@gmail.com   
      
   Dear Poutnik,   
      
   Thank you very much for this help!   
   And if I don't want to buy these tablets but prepare it from borax or Boric   
   Acid ??   
      
   Thank you very much again,   
   Ashi   
      
   On Monday, September 25, 2017 at 7:57:51 PM UTC+2, Poutnik wrote:   
   > Dne 22/09/2017 v 12:21 Ashu Seq napsal(a):   
   > > Dear all,   
   > > I want to make a  50mM Borate buffered Saline pH 8.4 (150 mM NaCl). Could   
   you please advise me the best way. Thank you!   
   > > Best wishes,   
   > > Ashu   
   > >   
   > 1/ Buy it.   
   >   
   > 2/ There are tables for laboratory buffer preparation. Look for it   
   >   in a good library or internet if you find it.   
   >   
   > 3/ Prepare it from dissolved 150 mM NaCl   
   >    and 50 mM in near 1 L of water   
   >    AND adjusting the pH by concentrated solution of sodium hydroxide   
   >    with help of calibrated pH meter.   
   >   
   >   
   > --   
   > Poutnik ( The Pilgrim, Der Wanderer )   
   >   
   > A wise man guards words he says,   
   > as they say about him more,   
   > than he says about the subject.   
      
   --- SoupGate-Win32 v1.05   
    * Origin: you cannot sedate... all the things you hate (1:229/2)   

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