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   sci.chem      Chemistry and related sciences      55,615 messages   

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   Message 55,211 of 55,615   
   Treon Verdery to All   
   Seeing inside a motile fertile sperm, or   
   30 Sep 22 06:03:58   
   
   From: treon3verdery@gmail.com   
      
   Seeing inside a motile fertile sperm, or also eggs, scanning the DNA at the   
   chromatin benefits beneficial eugenics; the quantum camera as described at new   
   scientist magazine ( article contains the words "figurine" and "spin") can see   
   light that does not    
   have a return pathway (no far side sensors, light diffusion and scattering, no   
   mirrors), the light can just go into the chromatin and be absorbed, a few   
   thousand words on at this note I write about ways a light microscope could   
   have resolving power/   
   resolution less than 1 nanometer, if that works then combining it with a   
   quantum camera shows some of what is going on with the chromatin of the sperm,   
   DNA reading could be possible by attaching single stranded DNA (or some other   
   thing that matches DNA)    
   to a quantum dot and using a UV quantum camera to see the quantum dots that   
   are matched to which DNA, quantum dots of 20-120 atoms might be particularly   
   good at diffusing into and throughout chromatin at sperm and eggs   
      
   Quantum dots with two energy levels simultaneously that do new things; silver   
   has two mobile electrons rather than just one, so a silver containing quantum   
   dot like ZnSeAg, or if it possible AgSe(AgS is a semiconductor) has some kind   
   of two electron    
   effect which may be useful; also, organic quantum dots already exist, but/and   
   (niftily) there are two electron systems/molecules like chlorophyll that   
   exist; to keep the size near 50 atoms maybe donut shaped chlorophyll (side   
   edges connected to make    
   overall toroidal molecule) or polymers or stuffed olives (Qd with leaky   
   chlorophyll polymer core) could be quantum dots; some quantum dots are coated   
   so it could be possible to coat toroidal chlorophyll or other 2 electron   
   system organic chemical quantum    
   dots with a long life coating, the coating on the organic quantum dot could   
   even possibly be "connected" to the organic quantum dot with the same kind of   
   hydrogen bonding that connects half-rungs of DNA together   
      
   Spin trap molecule quantum dots, there are organic chemical spin trap   
   molecules (PBN is one),and organic quantum dots, if a quantum dot can be made   
   from, or have spin trap attributes then because of the Pauli exclusion   
   principle and bandgap things spin    
   trap quantum dots may do something completely new (magnetic?; narrower   
   spectral output; atom based logic (perhaps spin up or down; for example at a   
   silver atom (2e stem) there could be 4 possible spin configurations from   
   attaching, or nearnessizing Ag or    
   AgSe or just pure Ag atom to a spin trap)   
      
   Could a 50 atom - 10nM dendritic polymer of an organic chemical be a quantum   
   dot? like maybe donut shaped chlorophyll polymer (side edges connected to make   
   overall toroidal molecule ); organic quantum dots already exist   
      
   Using quantum dots to see inside sperm; Nubby primer like 1/2 DNA ladder or a   
   4 bases could be all very loose wobble codons so the QD-matching -nucleotide   
   of new form could glom to the supercoiled DNA in sperm chromatin but come off   
   easily so as to omit    
   risk at the sperm which is being characterized so it can be used at actual   
   fertilization; quantum dot attached to oligonucleotide sequences have already   
   been used to detect DNA sequences in a human gene, as a technology washing a   
   plate with 100k sperm on    
   it (and using harmless osmosis and transport enhancers)builds up different   
   simultaneous spectra emissions and if there is a sperm with say all 8   
   preferred genes you can spot that sperm with flow cytometry (UV light at the   
   flow cytometer makes the matched    
   DNA quantum dots fluoresce)   
      
   You can also just make the diploid chromosomes at the tissue culture that   
   grows sperm to be identical and genetically enhanced or also genetically   
   optimized, any of the sperm have identically wonderful and beneficial genes   
      
   I read they can grow fertile motile mouse sperm at tissue culture, these are   
   technologies that may assist with tissue culture of genetically enhanced as   
   well as genetically optimized sperm:   
      
   MRNA at missing step; what is the mRNA profile between healthy human spermatid   
   to swimming spermatozooa, at human sperm tissue culture is the messenger RNA   
   profile different   
      
   Has anyone tried sonicating (blenderizing) healthy spermatids, fractionating   
   the results and then adding the "well spermatid" liquid/solid fractions to   
   human sperm tissue culture at a 3:1 ratio?   
      
   Double juice 99th percentile is what I describe as finding humans with double   
   the median amount of sperm production, then doing mRNA profiling of their   
   sperm generating tissues, possibly even at the spermatid stage then comparing   
   their mRNA profile to    
   the IPS (induced pluripotent cells) tissue culture spermatids; then the tissue   
   culture spermatids or other cell types on the path to spermatozoa are given   
   RNA drugs to mimic the high efficiency sperm generating tissue and high   
   efficiency spermatid    
   profile;    
      
   Male fetus testis; male 14 year old testis, this is extra ignorant but many   
   tissue implants prosper and youthify when implanted into much younger tissues   
   and bodies, it is possible using chimpanzee fetuses to remove and culture   
   prenatal testicular tissue,   
    raising human sperm in that, with the medium supplemented with testosterone   
   (among other things), if that works then an all-human system based on tissue   
   culture of aborted or preterm stillbirth is better to utilize; also, at the   
   source of the fetal    
   tissue it is beneficial due to epigenetic effects to gather fetal tissues from   
   parents at the 99th percentile of intelligence (g, like IQ) and the 95th   
   percentile of subjective well being (happiness) places like MIT, Caltech,   
   Stanford, Oxford are places    
   to find the women that frequently fit these criteria    
      
   It is possible that existing tissue culture of sperm already works, it is just   
   a one per 100k or million spermatids making spermatozooa (human spermatid   
   culture is previously accomplished) that develop to make motile fertile sperm;   
   using flow cytometry    
   of a tissue culture (gentle) homogenate they could find anything with a tail,   
   if they find some then those protocols could be improved;   
      
   There is a chance spermatids will grow at *liquid* tissue culture from induced   
   pluripotent stem cells (IPS), then using 96 well plates and swimming sperm   
   specific dyes 10,000 growth media variations can be tried with automated micro   
   pipetting/   
   microfluidics to get chemical facts on what gets them to the motile, fertile   
   spermatazooa stage   
      
      
   [continued in next message]   
      
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    * Origin: you cannot sedate... all the things you hate (1:229/2)   

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