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|    sci.chem    |    Chemistry and related sciences    |    55,615 messages    |
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|    Message 55,211 of 55,615    |
|    Treon Verdery to All    |
|    Seeing inside a motile fertile sperm, or    |
|    30 Sep 22 06:03:58    |
      From: treon3verdery@gmail.com              Seeing inside a motile fertile sperm, or also eggs, scanning the DNA at the       chromatin benefits beneficial eugenics; the quantum camera as described at new       scientist magazine ( article contains the words "figurine" and "spin") can see       light that does not        have a return pathway (no far side sensors, light diffusion and scattering, no       mirrors), the light can just go into the chromatin and be absorbed, a few       thousand words on at this note I write about ways a light microscope could       have resolving power/       resolution less than 1 nanometer, if that works then combining it with a       quantum camera shows some of what is going on with the chromatin of the sperm,       DNA reading could be possible by attaching single stranded DNA (or some other       thing that matches DNA)        to a quantum dot and using a UV quantum camera to see the quantum dots that       are matched to which DNA, quantum dots of 20-120 atoms might be particularly       good at diffusing into and throughout chromatin at sperm and eggs              Quantum dots with two energy levels simultaneously that do new things; silver       has two mobile electrons rather than just one, so a silver containing quantum       dot like ZnSeAg, or if it possible AgSe(AgS is a semiconductor) has some kind       of two electron        effect which may be useful; also, organic quantum dots already exist, but/and       (niftily) there are two electron systems/molecules like chlorophyll that       exist; to keep the size near 50 atoms maybe donut shaped chlorophyll (side       edges connected to make        overall toroidal molecule) or polymers or stuffed olives (Qd with leaky       chlorophyll polymer core) could be quantum dots; some quantum dots are coated       so it could be possible to coat toroidal chlorophyll or other 2 electron       system organic chemical quantum        dots with a long life coating, the coating on the organic quantum dot could       even possibly be "connected" to the organic quantum dot with the same kind of       hydrogen bonding that connects half-rungs of DNA together              Spin trap molecule quantum dots, there are organic chemical spin trap       molecules (PBN is one),and organic quantum dots, if a quantum dot can be made       from, or have spin trap attributes then because of the Pauli exclusion       principle and bandgap things spin        trap quantum dots may do something completely new (magnetic?; narrower       spectral output; atom based logic (perhaps spin up or down; for example at a       silver atom (2e stem) there could be 4 possible spin configurations from       attaching, or nearnessizing Ag or        AgSe or just pure Ag atom to a spin trap)              Could a 50 atom - 10nM dendritic polymer of an organic chemical be a quantum       dot? like maybe donut shaped chlorophyll polymer (side edges connected to make       overall toroidal molecule ); organic quantum dots already exist              Using quantum dots to see inside sperm; Nubby primer like 1/2 DNA ladder or a       4 bases could be all very loose wobble codons so the QD-matching -nucleotide       of new form could glom to the supercoiled DNA in sperm chromatin but come off       easily so as to omit        risk at the sperm which is being characterized so it can be used at actual       fertilization; quantum dot attached to oligonucleotide sequences have already       been used to detect DNA sequences in a human gene, as a technology washing a       plate with 100k sperm on        it (and using harmless osmosis and transport enhancers)builds up different       simultaneous spectra emissions and if there is a sperm with say all 8       preferred genes you can spot that sperm with flow cytometry (UV light at the       flow cytometer makes the matched        DNA quantum dots fluoresce)              You can also just make the diploid chromosomes at the tissue culture that       grows sperm to be identical and genetically enhanced or also genetically       optimized, any of the sperm have identically wonderful and beneficial genes              I read they can grow fertile motile mouse sperm at tissue culture, these are       technologies that may assist with tissue culture of genetically enhanced as       well as genetically optimized sperm:              MRNA at missing step; what is the mRNA profile between healthy human spermatid       to swimming spermatozooa, at human sperm tissue culture is the messenger RNA       profile different              Has anyone tried sonicating (blenderizing) healthy spermatids, fractionating       the results and then adding the "well spermatid" liquid/solid fractions to       human sperm tissue culture at a 3:1 ratio?              Double juice 99th percentile is what I describe as finding humans with double       the median amount of sperm production, then doing mRNA profiling of their       sperm generating tissues, possibly even at the spermatid stage then comparing       their mRNA profile to        the IPS (induced pluripotent cells) tissue culture spermatids; then the tissue       culture spermatids or other cell types on the path to spermatozoa are given       RNA drugs to mimic the high efficiency sperm generating tissue and high       efficiency spermatid        profile;               Male fetus testis; male 14 year old testis, this is extra ignorant but many       tissue implants prosper and youthify when implanted into much younger tissues       and bodies, it is possible using chimpanzee fetuses to remove and culture       prenatal testicular tissue,        raising human sperm in that, with the medium supplemented with testosterone       (among other things), if that works then an all-human system based on tissue       culture of aborted or preterm stillbirth is better to utilize; also, at the       source of the fetal        tissue it is beneficial due to epigenetic effects to gather fetal tissues from       parents at the 99th percentile of intelligence (g, like IQ) and the 95th       percentile of subjective well being (happiness) places like MIT, Caltech,       Stanford, Oxford are places        to find the women that frequently fit these criteria               It is possible that existing tissue culture of sperm already works, it is just       a one per 100k or million spermatids making spermatozooa (human spermatid       culture is previously accomplished) that develop to make motile fertile sperm;       using flow cytometry        of a tissue culture (gentle) homogenate they could find anything with a tail,       if they find some then those protocols could be improved;              There is a chance spermatids will grow at *liquid* tissue culture from induced       pluripotent stem cells (IPS), then using 96 well plates and swimming sperm       specific dyes 10,000 growth media variations can be tried with automated micro       pipetting/       microfluidics to get chemical facts on what gets them to the motile, fertile       spermatazooa stage                     [continued in next message]              --- SoupGate-Win32 v1.05        * Origin: you cannot sedate... all the things you hate (1:229/2)    |
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